3 articles published in JoVE
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons Melanie Martin1,2, Ke Li1, Megan C. Wright2, Angelo C. Lepore1 1Department of Neuroscience, Farber Institute for Neurosciences, Sidney Kimmel Medical College at Thomas Jefferson University, 2Department of Biology, Arcadia University Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates Trevor Cross1, Courtney Schoff2, Dylan Chudoff3, LIbby Graves3, Haley Broomell4, Katrina Terry4, Jennifer Farina5, Alexandra Correa5, David Shade5, David Dunbar5 1Biology Department, University of the Sciences, 2Biology Department, Arcadia University, 3Biology Department, Immaculata University, 4Biology/Clinical Laboratory Science, Neumann University, 5Science Department, Cabrini College We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates.
Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice Megan Wright1, Amy Kim2, Young-Jin Son3 1Biology Department, Arcadia University, 2Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, 3Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.