3 articles published in JoVE
Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel Asako Tajima1, Wen Liu2, Isha Pradhan1, Suzanne Bertera1, Robert A. Lakomy1, William A. Rudert1, Massimo Trucco1,3, Wilson S. Meng2, Yong Fan1,3 1Institute of Cellular Therapeutics, Allegheny Health Network, 2Division of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University, 3Department of Biological Sciences, Carnegie Mellon University This video demonstrates a protocol to enrich thymic epithelial cells (TECs) with density gradient for FACS isolation. It also shows the use of EAK16-II/EAKIIH6 peptides to promote the TEC aggregate formation. The microenvironments of EAK16-II/EAKIIH6 hydrogel provide the 3-D configuration necessary to maintain the survival and function of the TECs.
Viability Assays for Cells in Culture Jessica M. Posimo1, Ajay S. Unnithan1, Amanda M. Gleixner1, Hailey J. Choi1, Yiran Jiang1, Sree H. Pulugulla1, Rehana K. Leak1 1Division of Pharmaceutical Sciences, Mylan School of Pharmacy, Duquesne University Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
A Tactile Automated Passive-Finger Stimulator (TAPS) Daniel Goldreich1,2, Michael Wong2, Ryan M. Peters2, Ingrid M. Kanics1 1Department of Occupational Therapy, Duquesne University, 2Department of Psychology, Neuroscience and Behaviour, McMaster University We describe a computer-controlled device for investigating the sense of touch: the Tactile Automated Passive-finger Stimulator (TAPS). We describe the components of TAPS, and show how TAPS is used to administer a two-interval forced-choice tactile grating orientation test.