Einstein Healthcare Network 1 article published in JoVE Behavior Using a Split-belt Treadmill to Evaluate Generalization of Human Locomotor Adaptation Erin V.L. Vasudevan1,2, Rami J. Hamzey1,2, Eileen M. Kirk2 1Physical Therapy, School of Health Technology and Management, Stony Brook University, 2Motor Learning Lab, Moss Rehabilitation Research Institute, Einstein Healthcare Network We describe a protocol for investigating human locomotor adaptation using the split-belt treadmill, which has two belts that can drive each leg at a different speed. We specifically focus on a paradigm designed to test the generalization of adapted locomotor patterns to different walking contexts (e.g., gait speeds, walking environments).