University of the Sciences 5 articles published in JoVE Neuroscience Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster Kirby M. Donnelly1, Margaret M. P. Pearce1 1Department of Biological Sciences, University of the Sciences Accumulating evidence supports the idea that pathogenic protein aggregates associated with neurodegenerative diseases spread between cells with prion-like properties. Here, we describe a method that enables visualization of cell-to-cell spreading of prion-like aggregates in the model organism, Drosophila melanogaster. Developmental Biology Stimulation of Notch Signaling in Mouse Osteoclast Precursors Gurpreet Kaur1,2, Jaimo Ahn1, Kurt D. Hankenson1,3, Jason W. Ashley1,4 1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors. Biology High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells Alexander Braun1, Nicole M. Caesar1, Kyvan Dang1, Kenneth A. Myers1 1Department of Biological Sciences, University of the Sciences in Philadelphia Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed. Environment An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates Trevor Cross1, Courtney Schoff2, Dylan Chudoff3, LIbby Graves3, Haley Broomell4, Katrina Terry4, Jennifer Farina5, Alexandra Correa5, David Shade5, David Dunbar5 1Biology Department, University of the Sciences, 2Biology Department, Arcadia University, 3Biology Department, Immaculata University, 4Biology/Clinical Laboratory Science, Neumann University, 5Science Department, Cabrini College We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates. Engineering Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics Sergio L. S. Freire1, Nathaniel Thorne1, Michael Wutkowski1, Selina Dao1 1Department of Mathematics, Physics and Statistics, University of the Sciences The protocol for fabrication and operation of field dewetting devices (Field-DW) is described, as well as the preliminary studies of the effects of electric fields on droplet contents.
