2 articles published in JoVE
Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster Kirby M. Donnelly1, Margaret M. P. Pearce1 1Department of Biological Sciences, University of the Sciences Accumulating evidence supports the idea that pathogenic protein aggregates associated with neurodegenerative diseases spread between cells with prion-like properties. Here, we describe a method that enables visualization of cell-to-cell spreading of prion-like aggregates in the model organism, Drosophila melanogaster.
Stimulation of Notch Signaling in Mouse Osteoclast Precursors Gurpreet Kaur1,2, Jaimo Ahn1, Kurt D. Hankenson1,3, Jason W. Ashley1,4 1Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, 2Department of Biological Sciences, University of the Sciences, 3The Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 4Department of Biology, College of Science, Technology, Engineering, & Mathematics, Eastern Washington University Notch signaling is a form of cellular communication that relies upon direct contact between cells. To properly induce Notch signaling in vitro, Notch ligands must be presented to cells in an immobilized state. This protocol describes methods for in vitro stimulation of Notch signaling in mouse osteoclast precursors.