2 articles published in JoVE
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma Phillip Huyett1, Mark Gilbert1, Lijun Liu2, Robert L. Ferris2, Seungwon Kim1 1Department of Otolaryngology, University of Pittsburgh Medical Center, 2University of Pittsburgh Cancer Institute, Hillman Cancer Center Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.
In vivo Optogenetic Stimulation of the Rodent Central Nervous System Michelle M. Sidor1, Thomas J. Davidson2, Kay M. Tye3, Melissa R. Warden4, Karl Diesseroth2,5, Colleen A. McClung1 1Department of Psychiatry, University of Pittsburgh Medical Center, 2Department of Bioengineering, Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior, Cornell University, 5Department of Psychiatry and Behavioral Sciences, Stanford University Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.