Warren Alpert Medical School of Brown University 6 articles published in JoVE Neuroscience Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron Geoff Stilwell1, Anthony Agudelo*1,2 1Department of Biology, Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital, Warren Alpert Medical School of Brown University We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg. Neuroscience Simultaneous Application of Transcranial Direct Current Stimulation during Virtual Reality Exposure Mascha van 't Wout-Frank1,2,3, Noah S. Philip1,2,3 1VA RR&D Center for Neurorestoration and Neurotechnology, Providence VA Medical Healthcare System, 2Department of Psychiatry and Human Behavior, Alpert Brown Medical School, 3COBRE Center for Neuromodulation, Butler Hospital This manuscript outlines a novel protocol to allow the simultaneous application of transcranial direct current stimulation during exposure to warzone trauma-related cues using virtual reality for veterans with posttraumatic stress disorder. Neuroscience Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions Benedetta Assetta*1, Changyong Tang*1,2, Jing Bian*3, Ryan O'Rourke1, Kevin Connolly1, Thomas Brickler3, Sundari Chetty3,4, Yu-Wen Alvin Huang1,5,6 1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University The neuron-glial interactions in neurodegeneration are not well understood due to inadequate tools and methods. Here, we describe optimized protocols to obtain induced neurons, oligodendrocyte precursor cells, and oligodendrocytes from human pluripotent stem cells and provide examples of the values of these methods in understanding cell-type-specific contributions in Alzheimer’s disease. Medicine Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction Karla Reichert*1, Bonnie Colantuono*1, Isabella McCormack1, Fernanda Rodrigues1, Vasile Pavlov1, M. Ruhul Abid1 1Cardiothoracic Surgery Division, Rhode Island Hospital, Brown University Warren Alpert Medical School We provide a reliable method for left anterior descending artery (LAD) ligation in a mouse model. This method is comparatively less invasive than other methods, involving endotracheal intubation, a left-sided thoracotomy approach, and thoracentesis. This method can be used as a model for both acute and chronic myocardial infarction (MI). Immunology and Infection SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells Collins Cheruiyot1, Zemplen Pataki1, Robert Williams1, Bharat Ramratnam2,3, Ming Li3 1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories, Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine, Warren Alpert Medical School, Brown University Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions. Medicine Developing Neuroimaging Phenotypes of the Default Mode Network in PTSD: Integrating the Resting State, Working Memory, and Structural Connectivity Noah S. Philip1, S. Louisa Carpenter1, Lawrence H. Sweet2 1Providence VA Medical Center, Department of Psychiatry & Human Behavior, Alpert Medical School, Brown University, 2Department of Psychology, University of Georgia This protocol describes the complementary neuroimaging techniques of resting state structural connectivity, task-induced deactivation, and structural connectivity analyses to examine the default network in post-traumatic stress disorder. The use of synergistic methods could potentially lead to improved diagnostics and assessments of severity, outcome, and other relevant clinical factors.
