2 articles published in JoVE
Live Cell Imaging during Mechanical Stretch Gabriel Rápalo1,2, Josh D. Herwig3, Robert Hewitt4, Kristina R. Wilhelm1,2, Christopher M. Waters1,2, Esra Roan3 1Department of Physiology, University of Tennessee Health Science Center, 2Department of Biomedical Engineering and Imaging, University of Tennessee Health Science Center, 3Department of Biomedical Engineering, University of Memphis, 4Department of Engineering Technology, University of Memphis A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters Chidambaram Ramanathan1, Sanjoy K. Khan1, Nimish D. Kathale1, Haiyan Xu1, Andrew C. Liu1 1Department of Biological Sciences, The University of Memphis Circadian clocks function within individual cells, i.e., they are cell-autonomous. Here, we describe methods for generating cell-autonomous clock models using non-invasive, luciferase-based real-time bioluminescence technology. Reporter cells provide tractable, functional model systems for studying circadian biology.