Department of Veterans Affairs Tennessee Valley 1 article published in JoVE Developmental Biology Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption Joseph M. Elsakr1, Charles Deeter2, Valerie Ricciardi3, Maureen Gannon1,4,5,6 1Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences, Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine, Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology, Vanderbilt University This protocol demonstrates the accurate and reproducible measurement of oxygen consumption in non-human primate pancreatic islets. The islet loading techniques and coating of the microplate provide a framework for efficient measurement of respiration in other types of cultured spheroids.
