Molecular Pathology Laboratory Network, Inc View Institution's Website 3 articles published in JoVE Biology Freezing, Thawing, and Packaging Cells for Transport Richard Ricardo1, Katy Phelan1 1Molecular Pathology Laboratory Network, Inc Cultured mammalian cells are used extensively in cell biology studies. This video describes the basic skills required to freeze and store cells and how to recover frozen stocks. Biology Counting and Determining the Viability of Cultured Cells Richard Ricardo1, Katy Phelan1 1Molecular Pathology Laboratory Network, Inc Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer. Biology Trypsinizing and Subculturing Mammalian Cells Richard Ricardo1, Katy Phelan1 1Molecular Pathology Laboratory Network, Inc As cells reach confluency, they must be subcultured or passaged. This video will demonstrate a procedure for subculturing both adherent and suspension cells.