3 articles published in JoVE
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs Maran L. Sprouse1, Gabriele Blahnik2, Thomas Lee1, Natalie Tully1, Pinaki Benarjee3, Eddie A. James2, Maria J. Redondo4, Matthew L. Bettini1, Maria Bettini1 1 The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue Kevin Ung1, Benjamin R. Arenkiel1,2,3 1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Department of Neuroscience, Baylor College of Medicine (BCM), 3Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital The development of optogenetics now provides the means to precisely stimulate genetically defined neurons and circuits, both in vitro and in vivo. Here we describe the assembly and implantation of a fiber optic for chronic photostimulation of brain tissue.
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices Jennifer Selever1, Jian-Qiang Kong2, Benjamin R. Arenkiel3,4 1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.