University of Texas Health Science Center at San Antonio

3 articles published in JoVE

• Neuroscience

  
  In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia John Shannonhouse¹, Ruben Gomez¹, Hyeonwi Son¹, Yan Zhang¹, Yu Shin Kim^{1,2 1}Department of Oral & Maxillofacial Surgery, School of Dentistry, University of Texas Health Science Center at San Antonio, ²Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences, University of Texas Health Science Center at San Antonio This protocol describes the surgical exposure of the dorsal root ganglion (DRG) followed by GCaMP3 (genetically-encoded Ca²⁺ indicator; Green Fluorescent Protein-Calmodulin-M13 Protein 3) Ca²⁺ imaging of the neuronal ensembles using Pirt-GCaMP3 mice while applying a variety of stimuli to the ipsilateral hind paw.

• Medicine

  
  Quality-Controlled Sputum Analysis by Flow Cytometry Marcia Grayson*¹, Shao-Chiang Lai*¹, Lydia H. Bederka¹, Patricia Araujo¹, Jamila Sanchez¹, Xavier T. Reveles¹, Vivienne I. Rebel^1,2, Jennifer Rebeles^{1 1}bioAffinity Technologies, ²Department of Cell Systems & Anatomy, The University of Texas Health Science Center at San Antonio This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.

• Immunology and Infection

  
  Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells Ziming Cheng¹, Ting Zhou^1,2, Azhar Merchant¹, Thomas J. Prihoda³, Brian L. Wickes^4,5, Guogang Xu², Christi A. Walter^2,5, Vivienne I. Rebel^{1,2,5 1}Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, ²Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, ³Department of Pathology, UT Health Science Center at San Antonio, ⁴Department of Microbiology, UT Health Science Center at San Antonio, ⁵Cancer Therapy and Research Center, UT Health Science Center at San Antonio Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.