2 articles published in JoVE
Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation Magdalena Delgado*1, Anisha Kothari*1, Walter N. Hittelman2, Timothy C. Chambers1 1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center This protocol describes the use of centrifugal elutriation to separate primary acute lymphoblastic leukemia cells into different cell cycle phases.
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation Pingyu Zhang*1, Andres Rojas*1, Boris Blechacz1 1Department of Gastroenterology, Hepatology and Nutrition, The University of Texas MD Anderson Cancer Center DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.