3 articles published in JoVE
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2 Desarey Morales Vasquez1, Kevin Chiem1, Jesus Silvas1, Jun-Gyu Park1, Chengjin Ye1, Luis Martínez-Sobrido1 1Texas Biomedical Research Institute This protocol describes the dynamics of viral infections using luciferase- and fluorescence-expressing recombinant (r)SARS-CoV-2 and an in vivo imaging systems (IVIS) in K18 hACE2 transgenic mice to overcome the need of secondary approaches required to study SARS-CoV-2 infections in vivo.
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells Hector Guillen-Ahlers1,2, Prahlad K. Rao1, Danu S. Perumalla1, Maria J. Montoya1, Avinash Y.L. Jadhav1, Michael R. Shortreed3, Lloyd M. Smith3, Michael Olivier1,2 1Department of Genetics, Texas Biomedical Research Institute, 2Department of Internal Medicine-Molecular Medicine, Wake Forest University School of Medicine, 3Department of Chemistry, University of Wisconsin This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.
Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat Carmen Hinojosa-Laborde1, Brian Jespersen2, Robert Shade3 1Tactical Combat Casualty Care Research, U.S. Army Institute of Surgical Research, 2Department of Pharmacology and Toxicology, Michigan State University, 3Southwest National Primate Research Center, Texas Biomedical Research Institute The purpose of this protocol is to demonstrate the principles and techniques for measuring and calculating glomerular filtration rate, urine flow rate, and excretion of sodium and potassium in a rat. This demonstration can be used to provide students with an overall conceptual understanding of how to measure renal function.