3 articles published in JoVE
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture Ritika Tewari*1, Savannah J. West*1,2, Bieerkehazi Shayahati1, Askar M. Akimzhanov1 1Department of Biochemistry and Molecular Biology, McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.
Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits Andrew D. Sligar1, Gretchen Howe2, Julia Goldman3, Patricia Felli2, Varsha Karanam1, Richard W. Smalling2,4, Aaron B. Baker1,5,6,7 1Department of Biomedical Engineering, University of Texas at Austin, 2Division of Cardiology, University of Texas McGovern Medical School, 3Center for Laboratory Animal Medicine and Care, UT Health Science Center at Houston, 4Memorial Herman Heart and Vascular Center, Texas Medical Center, 5Institute for Cellular and Molecular Biology, University of Texas at Austin, 6The Institute for Computational Engineering and Sciences, University of Texas at Austin, 7Institute for Biomaterials, Drug Delivery and Regenerative Medicine, University of Texas at Austin We describe a surgical procedure used to induce peripheral ischemia in rabbits with hyperlipidemia and diabetes. This surgery acts as a preclinical model for conditions experienced in peripheral artery disease in patients. Angiography is also described as a means to measure the extent of introduced ischemia and recovery of perfusion.
Technique to Target Microinjection to the Developing Xenopus Kidney Bridget D. DeLay1, Vanja Krneta-Stankic1,2, Rachel K. Miller1,2,3,4 1Department of Pediatrics, Pediatric Research Center, University of Texas McGovern Medical School, 2Program in Genes & Development, University of Texas Graduate School of Biomedical Sciences, 3Program in Cell & Regulatory Biology, University of Texas Graduate School of Biomedical Sciences, 4Department of Genetics, University of Texas MD Anderson Cancer Center Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.