3 articles published in JoVE
A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome Piotr Przanowski1, Zeming Zheng1, Urszula Wasko1, Sanchita Bhatnagar1,2 1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, 2Department of Neuroscience, University of Virginia School of Medicine Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.
A Cell Culture Model of Resistance Arteries Lauren A. Biwer1,2, Christophe Lechauve3, Sheri Vanhoose4, Mitchell J. Weiss3, Brant E. Isakson1,2 1Department of Molecular Physiology and Biophysics, University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, 3 A cell culture model of resistance arteries is described, allowing for the dissection of signaling pathways in endothelium, smooth muscle, or between endothelium and smooth muscle (the myoendothelial junction). The selective application of agonists or protein isolation, electron microscopy, or immunofluorescence can be utilized using this cell culture model.
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster Michelle L. Bland1 1Department of Pharmacology, University of Virginia This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.