2 articles published in JoVE
Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response John A. Richey1, Holly Sullivan-Toole2, Marlene Strege1, Corinne Carlton1, Dylan McDaniel3, Matthew Komelski4, Amy Epperley5, Hongxiao Zhu6, Irving C. Allen3,7 1Department of Psychology, Virginia Tech, 2Graduate Program in Translational Biology, Medicine and Health, Virginia Tech, 3Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 4Department of Human Development and Family Science, Virginia Tech, 5Wellness Center, Virginia Tech, 6Department of Statistics, Virginia Tech, 7Department of Basic Science Education, Virginia Tech Carilion School of Medicine Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory response.
Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy Konark Mukherjee1, Helen R. Clark1, Vrushali Chavan1, Emily K. Benson2, Grahame J. Kidd2, Sarika Srivastava1 1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.