VA Puget Sound 3 articles published in JoVE Medicine Laser Doppler Perfusion Imaging in the Mouse Hindlimb Gale L. Tang1,2, Kevin J. Kim2 1Division of Vascular Surgery, University of Washington, 2VA Puget Sound Health Care System Here, we present a protocol that demonstrates the technique and necessary controls for Laser Doppler perfusion imaging to measure blood flow in the mouse hindlimb. Behavior Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze Marcella M. Cline1, Megan A. Ostlie2, Chloe G. Cross3, Gregory G. Garwin2, Satoshi Minoshima2, Donna J. Cross2 1Department of Radiology, University of Washington, 2Department of Radiology, University of Utah, 3Geriatric Research Education and Clinical Center (GRECC), VA Puget Sound Here we present a protocol for a mouse-specific test of cognition that does not require swimming. This test can be used to successfully distinguish controlled cortical impact-induced traumatic brain injury mice from sham controls. Behavior Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise Jennifer D. Conner1,2, Tami Wolden-Hanson1, LeBris S. Quinn1,3,4 1Research Service, VA Puget Sound Health Care System, 2Seattle Institute for Biomedical and Clinical Research, 3Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, 4Geriatric Research, Education, and Clinical Center, VA Puget Sound Health Care System A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.
