2 articles published in JoVE
Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications Michal Adamski1, Gianluca Fontana2, Joshua R. Gershlak4, Glenn R. Gaudette4, Hau D. Le1, William L. Murphy2,3 1Department of Surgery, University of Wisconsin-Madison, 2Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, 3Department of Biomedical Engineering, University of Wisconsin College of Engineering, 4Department of Biomedical Engineering, Worcester Polytechnic Institute Here we present, and contrast two protocols used to decellularize plant tissues: a detergent-based approach and a detergent-free approach. Both methods leave behind the extracellular matrix of the plant tissues used, which can then be utilized as scaffolds for tissue engineering applications.
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes Megan E. Dowdle1, Susanne Blaser Imboden1, Sookhee Park1, Sean P. Ryder2, Michael D. Sheets1 1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 2Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.