University of Wisconsin College of Engineering 1 article published in JoVE Bioengineering Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications Michal Adamski1, Gianluca Fontana2, Joshua R. Gershlak4, Glenn R. Gaudette4, Hau D. Le1, William L. Murphy2,3 1Department of Surgery, University of Wisconsin-Madison, 2Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, 3Department of Biomedical Engineering, University of Wisconsin College of Engineering, 4Department of Biomedical Engineering, Worcester Polytechnic Institute Here we present, and contrast two protocols used to decellularize plant tissues: a detergent-based approach and a detergent-free approach. Both methods leave behind the extracellular matrix of the plant tissues used, which can then be utilized as scaffolds for tissue engineering applications.
