3 articles published in JoVE
A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy Xiaoliang Wang*1, Muhammad A. Chaudhry*2, Ying Nie2, Zijian Xie1, Joseph I. Shapiro2, Jiang Liu1,2 1Marshall Institute for Interdisciplinary Research (MIIR), Marshall University, 2Department of Biomedical Sciences, Marshall University Joan C. Edwards School of Medicine This manuscript provides a detailed two-step surgical procedure to perform mouse 5/6th partial nephrectomy (PNx) with pole ligation. Four weeks after surgery, in comparison with sham-operated mice, the PNx mice developed impaired renal function, anemia, cardiac hypertrophy, cardiac fibrosis, and decreased heart systolic and diastolic function.
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking Ashley M. Fox-Loe1, Brandon J. Henderson2, Christopher I. Richards1 1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University Labeling the extracellular domain of a membrane protein with a pH sensitive fluorophore, superecliptic pHluorin (SEP), allows subcellular localization, expression, and trafficking to be determined. Imaging SEP-labeled proteins with total internal reflection fluorescence microscopy (TIRFM) enables the quantification of protein levels in the peripheral ER and plasma membrane.
Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses Catherine L. Franssen1, Massimo Bardi2, Kelly G. Lambert1 1Department of Psychology, Randolph-Macon College, 2Department of Psychology, Marshall University The comparative species approach allows behavioral neuroscientists to explore various neurobiological factors associated with specific behaviors viewed as characteristic of a specific animal model. Taking advantage of naturally occurring differences in behavior between closely related species, this technique doesn’t require invasive techniques to manipulate the expression of the behavior.