Instituto Evandro Chagas 1 article published in JoVE Immunology and Infection Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates Adriana B. Almeida1, Perla F. Araújo1, Francisco M. Bernal1, Ana de Cassia Rosa1, Sebastião A. Valente2, Antonio R.L. Teixeira1 1Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, 2Instituto Evandro Chagas The Trypanosoma cruzi agent of Chagas disease produces long-lasting asymptomatic infections that abruptly develop into clinically recognized pathology. The following research protocol describes a short-run family-based epidemiological study to unravel the T. cruzi infection transmitted sexually from parent to progeny.