National Institute of Metrology, Quality and Technology (Inmetro) 1 article published in JoVE Bioengineering Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting Gabriela S. Kronemberger1,2,3, Guilherme A. S. C. Miranda1,2,4, Taisnara I. G. Silva1,2,4, Rosângela M. Gonçalves1,2, José M. Granjeiro2,3,4,5, Leandra S. Baptista1,2,3,4 1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches.
