Federal University of Pelotas 1 article published in JoVE Biology Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome Sarah R. Ocañas*1,2,3, José V. V. Isola*1,3, Tatiana D. Saccon1, Kevin D. Pham2, Ana J. Chucair-Elliott2, Augusto Schneider4, Willard M. Freeman2,3, Michael B. Stout1,3 1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.
