Universidad San Sebastian 2 articles published in JoVE Neuroscience Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein Daniela Elgueta1, Ornella Chovar1, Valentina Ugalde1, Vanesa Sánchez-Guajardo1, Alejandra Catenaccio2, Felipe Court2,3,4, Rodrigo Pacheco1,5 1Laboratorio de Neuroinmunología, Fundación Ciencia & Vida, 2Center for Integrative Biology, Universidad Mayor, 3FONDAP Geroscience Center for Brain Health and Metabolism, 4Buck Institute for Research on Ageing, 5Facultad de Medicina y Ciencia, Universidad San Sebastián This work analyzes the vector dose and exposure time required to induce neuroinflammation, neurodegeneration, and motor impairment in this preclinical model of Parkinson's disease. These vectors encoding the human α-synuclein are delivered into the substantia nigra to recapitulate the synuclein pathology associated with Parkinson's disease. Immunology and Infection Studying Organelle Dynamics in B Cells During Immune Synapse Formation Jorge Ibañez-Vega*1, Danitza Fuentes*1, Jonathan Lagos1,2, Jorge Cancino3, María Isabel Yuseff1 1Laboratory of Immune Cell Biology, Department of Cellular and Molecular Biology, Pontificia Universidad Católica de Chile, 2Faculty of Medicine, Pontificia Universidad Católica de Chile, 3Centro de Investigaciones en Biología Celular y Biomedicina, Facultad de Ciencia, Universidad San Sebastián Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
