Institut Pasteur de Montevideo 1 article published in JoVE Developmental Biology Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos Daniel Prieto1, Gonzalo Aparicio1,2, Matías Machado1, Flavio R. Zolessi1,2 1Institut Pasteur de Montevideo, 2Sección Biología Celular, Facultad de Ciencias, Universidad de la República A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
