Universidad de la Republica 6 articles published in JoVE Medicine High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa Pilar Irigoyen1, Rossana Sapiro1,3, Adriana Cassina2,3 1Departamento de Histología y Embriología, Unidad Académica Histología y Embriología, 2Departamento de Bioquímica, Unidad Académica Bioquímica, 3Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República The analysis of sperm mitochondrial function by high-resolution respirometry permits the measurement of the oxygen consumption of freely moving spermatozoa in a closed-chamber system. The technique can be applied to measure respiration in human spermatozoa, which provides information on sperm mitochondrial characteristics and integrity. Developmental Biology Polysome Purification from Soybean Symbiotic Nodules María Martha Sainz1, Carla Valeria Filippi1, Guillermo Eastman2,3, Mariana Sotelo-Silveira1, C. Mauro Martinez1, Omar Borsani1, José Sotelo-Silveira2,4 1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, 2Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology, University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels. Developmental Biology Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots Ines Rauschert*2, Juan Claudio Benech*2, Martha Sainz1, Omar Borsani1, Mariana Sotelo-Silveira1 1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), 2Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE) The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit). Neuroscience Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions Carmen Bolatto*1, Silvia Olivera-Bravo*2, Sofía Cerri1,2 1Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine, Universidad de la República, 2Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE), Ministerio de Educación y Cultura The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements. Developmental Biology Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos Daniel Prieto1, Gonzalo Aparicio1,2, Matías Machado1, Flavio R. Zolessi1,2 1Institut Pasteur de Montevideo, 2Sección Biología Celular, Facultad de Ciencias, Universidad de la República A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos. Medicine Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections Natalia Muñoz-Wolf1,2, Analía Rial1, José M. Saavedra1, José A. Chabalgoity1 1Departamento de Desarrollo Biotecnológico, Universidad de la República, 2Present Affiliation: Trinity Biomedical Science Institute, Trinity College Dublin The present work illustrates the convenience of using sublingual immunotherapy to boost the innate immune response in the lungs and confer protection against acute pneumococcal pneumonia in mouse.
