Instituto de Investigaciones Biologicas Clemente Estable (IIBCE) 3 articles published in JoVE Developmental Biology Polysome Purification from Soybean Symbiotic Nodules María Martha Sainz1, Carla Valeria Filippi1, Guillermo Eastman2,3, Mariana Sotelo-Silveira1, C. Mauro Martinez1, Omar Borsani1, José Sotelo-Silveira2,4 1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, 2Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology, University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels. Developmental Biology Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots Ines Rauschert*2, Juan Claudio Benech*2, Martha Sainz1, Omar Borsani1, Mariana Sotelo-Silveira1 1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), 2Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE) The atomic force microscopy indentation protocol offers the possibility to dissect the role of the physical properties of the cell wall of a particular cell of a tissue or organ during normal or constrained growth (i.e., under water deficit). Biology Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions Rosana Rodríguez-Casuriaga1, Gustavo A. Folle2, Federico Santiñaque2, Beatriz López-Carro2, Adriana Geisinger1,3 1Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 2Servicio de Citometría de Flujo y Clasificación Celular (SECIF), Instituto de Investigaciones Biológicas Clemente Estable, 3Sección Bioquímica, Facultad de Ciencias, Universidad de la República A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.
