Ministerio de Educacion y Cultura 1 article published in JoVE Neuroscience Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions Carmen Bolatto*1, Silvia Olivera-Bravo*2, Sofía Cerri1,2 1Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine, Universidad de la República, 2Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE), Ministerio de Educación y Cultura The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.
