Federal University of Rio De Janeiro

5 articles published in JoVE

• Bioengineering

  
  Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting Gabriela S. Kronemberger^1,2,3, Guilherme A. S. C. Miranda^1,2,4, Taisnara I. G. Silva^1,2,4, Rosângela M. Gonçalves^1,2, José M. Granjeiro^2,3,4,5, Leandra S. Baptista^{1,2,3,4 1}Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, ²Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), ³Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, ⁴Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), ⁵Dental School, Fluminense Federal Fluminense Here, we describe the large-scale production of adipose-derived stromal/stem cell (ASC) spheroids using an automated pipetting system to seed the cell suspension, thus ensuring homogeneity of spheroid size and shape. These ASC spheroids can be used as building blocks for 3D bioprinting approaches.

• Environment

  
  A Field Primer for Monitoring Benthic Ecosystems Using Structure-From-Motion Photogrammetry Ty N. F. Roach¹, Shreya Yadav¹, Carlo Caruso¹, Jenna Dilworth^1,2, Catherine M. Foley¹, Joshua R. Hancock¹, Joel Huckeba¹, Ariana S. Huffmyer¹, Kira Hughes¹, Valerie A. Kahkejian¹, Elizabeth M.P. Madin¹, Shayle B. Matsuda¹, Michael McWilliam¹, Spencer Miller^1,3, Erika P. Santoro⁴, Mariana Rocha de Souza¹, Damaris Torres-Pullizaa¹, Crawford Drury¹, Joshua S. Madin^{1 1}Hawai'i Institute of Marine Biology, University of Hawai'i Manoa, ²Rosenstiel School of Marine and Atmospheric Science, University of Miami, ³Hawai'i Pacific University, ⁴Federal University of Rio de Janeiro We provide a detailed protocol for conducting underwater structure-from-motion photogrammetry surveys to generate 3D models and orthomosaics.

• Bioengineering

  
  Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation Helena D. M. Villela¹, Caren L. S. Vilela¹, Juliana M. Assis¹, Natascha Varona², Camille Burke², David A. Coil², Jonathan A. Eisen², Raquel S. Peixoto^{1,2,3 1}LEMM, Laboratory of Molecular Microbial Ecology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (UFRJ), ²Genome Center, University of California, Davis, ³IMAM-AquaRio - Rio de Janeiro Aquarium Research Center Pollution affects all biomes. Marine environments have been particularly impacted, especially coral reefs, one of the most sensitive ecosystems on Earth. Bioremediation is the capacity of organisms to degrade contaminants. Here, we describe methodologies to isolate and test microbes presenting bioremediation ability and potential probiotic characteristics for corals.

• Bioengineering

  
  Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins Anna C. N. T. F. Corrêa¹, Patricia R. Pereira¹, Vânia M. F. Paschoalin^{1 1}Chemistry Institute, Federal University of Rio de Janeiro This study describes classical hydration using the thin lipid film method for nanoliposome preparation followed by nanoparticle characterization. A 47 kDa-hydrophilic and globular protein, tarin, is successfully encapsulated as a strategy to improve stability, avoid fast clearance, and promote controlled release. The method can be adapted to hydrophobic molecules encapsulation.

• Chemistry

  
  CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Rafael D. Melani*^1,2, Henrique S. Seckler*¹, Owen S. Skinner¹, Luis H. F. Do Vale^1,3, Adam D. Catherman¹, Pierre C. Havugimana¹, Marcelo Valle de Sousa³, Gilberto B. Domont², Neil L. Kelleher¹, Philip D. Compton^{1 1}Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, ²Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, ³Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.