3 articles published in JoVE
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies Ismael C. Gomes*1, Mariana Acquarone*1, Renata de Moraes Maciel*1, Rafael Bierig Erlich1, Stevens K. Rehen1 1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.
Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test Fabio S.L. da Conceição1, Stacie Ngo-Abdalla1, Jean-Christophe Houzel1, Stevens K. Rehen1 1Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brasil Parkinson disease is caused by loss of dopaminergic innervation to the striatum, which can be experimentally induced by 6-OH-dopamine. We describe how to perform a stereotaxic lesion and to monitor apomorphine-induced rotational behavior in mice. This model is useful and reliable for testing new therapies for Parkinson disease.
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping Priscila B. Campos1, Rafaela C. Sartore1, Stacie N. Abdalla1, Stevens K. Rehen1 1Institute of Biomedical Sciences, Federal University of Rio De Janeiro-UFRJ Karyotyping is a simple and useful technique widely used for detecting genetic alterations. Here we describe a step by step protocol for chromosome spread preparation of human embryonic stem cells for monitoring the chromosomal status of these cells maintained in culture.