3 articles published in JoVE
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds Calliandra Maria de-Souza-Silva1, Fernanda Guilhelmelli1, Daniel Zamith-Miranda2,3, Marco Antônio de Oliveira1, Joshua Daniel Nosanchuk2,3, Ildinete Silva-Pereira*1, Patrícia Albuquerque*1,4 1Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, 2Department of Microbiology and Immunology, Albert Einstein College of Medicine, 3Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, 4Faculty of Ceilândia, University of Brasília An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Rafael D. Melani*1,2, Henrique S. Seckler*1, Owen S. Skinner1, Luis H. F. Do Vale1,3, Adam D. Catherman1, Pierre C. Havugimana1, Marcelo Valle de Sousa3, Gilberto B. Domont2, Neil L. Kelleher1, Philip D. Compton1 1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.
Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model Antonio R. L. Teixeira1, Nadjar Nitz1, Francisco M. Bernal1, Mariana M. Hecht1 1Chagas Disease Multidisciplinary Research Laboratory, University of Brasilia The inoculation of Trypanosoma cruzi in fertile eggs prior to incubation renders the parasite kDNA minicircle integration in embryo cells genome. Crossbreeding reveals the vertical transfer of the mutations to progeny. The kDNA integrates into coding regions at several chromosomes and the chickens die with an inflammatory autoimmune heart disease.