University of Brasilia

4 articles published in JoVE

• Immunology and Infection

  
  Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates Adriana B. Almeida¹, Perla F. Araújo¹, Francisco M. Bernal¹, Ana de Cassia Rosa¹, Sebastião A. Valente², Antonio R.L. Teixeira^{1 1}Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasilia, ²Instituto Evandro Chagas The Trypanosoma cruzi agent of Chagas disease produces long-lasting asymptomatic infections that abruptly develop into clinically recognized pathology. The following research protocol describes a short-run family-based epidemiological study to unravel the T. cruzi infection transmitted sexually from parent to progeny.

• Biology

  
  Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds Calliandra Maria de-Souza-Silva¹, Fernanda Guilhelmelli¹, Daniel Zamith-Miranda^2,3, Marco Antônio de Oliveira¹, Joshua Daniel Nosanchuk^2,3, Ildinete Silva-Pereira*¹, Patrícia Albuquerque*^{1,4 1}Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, ²Department of Microbiology and Immunology, Albert Einstein College of Medicine, ³Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, ⁴Faculty of Ceilândia, University of Brasília An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.

• Chemistry

  
  CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis Rafael D. Melani*^1,2, Henrique S. Seckler*¹, Owen S. Skinner¹, Luis H. F. Do Vale^1,3, Adam D. Catherman¹, Pierre C. Havugimana¹, Marcelo Valle de Sousa³, Gilberto B. Domont², Neil L. Kelleher¹, Philip D. Compton^{1 1}Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, ²Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, ³Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.

• Immunology and Infection

  
  Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model Antonio R. L. Teixeira¹, Nadjar Nitz¹, Francisco M. Bernal¹, Mariana M. Hecht^{1 1}Chagas Disease Multidisciplinary Research Laboratory, University of Brasilia The inoculation of Trypanosoma cruzi in fertile eggs prior to incubation renders the parasite kDNA minicircle integration in embryo cells genome. Crossbreeding reveals the vertical transfer of the mutations to progeny. The kDNA integrates into coding regions at several chromosomes and the chickens die with an inflammatory autoimmune heart disease.