University of Campinas (UNICAMP) 7 articles published in JoVE Biology Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds Matheus Pena-Passos1, Laís Soêmis Sisti1, Juliana Lischka Sampaio Mayer1 1LabPlaM - Mycoheterotrophic Plants Research Lab, Department of Plant Biology, University of Campinas (UNICAMP) This protocol aims to provide detailed procedures for collecting, fixing, and maintaining mycoheterotrophic plant samples, applying different microscopy techniques such as scanning and transmission electron microscopy, light, confocal, and fluorescence microscopy to study fungal colonization in plants tissues and seeds germinated with mycorrhizal fungi. Chemistry An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment Talita Miguel Marin1, Nathalia de Carvalho Indolfo4, Silvana Aparecida Rocco1, Murilo de Carvalho1,2, Marilia Meira Dias1, Graziele Izalina Vasconcelos Bento1, Leandro Oliveira Bortot1, Desirée Cigaran Schuck3, Márcio Lorencini3, Eduardo Pagani1 1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), 2Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 3Grupo Boticário, 4Institute of Biology, University of Campinas We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results. Medicine Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies Rodrigo Menezes Forti1,2, Marilise Katsurayama2,3, Giovani Grisotti Martins1, Lenise Valler2,3, Andrés Quiroga1,2, Luiz Simioni1, Julien Menko4, Antonio L. E. Falcão3, Li Min Li2,5, Rickson C. Mesquita1,2 1Institute of Physics, University of Campinas, 2Brazilian Institute of Neuroscience and Neurotechnology, 3Clinical Hospital, University of Campinas, 4Department of Emergency Medicine, Albert Einstein College of Medicine, 5School of Medical Sciences, University of Campinas Presented here is a protocol for non-invasively monitoring cerebral hemodynamics of neurocritical patients in real-time and at the bedside using diffuse optics. Specifically, the proposed protocol uses a hybrid diffuse optical systems to detect and display real-time information on cerebral oxygenation, cerebral blood flow and cerebral metabolism. Chemistry Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products Renata Sigrist1, Bruno S. Paulo1, Célio F. F. Angolini2, Luciana G. De Oliveira1 1Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 2Center for Natural and Human Sciences, Federal University of ABC (UFABC) A mass spectrometry-guided genome mining protocol is established and described here. It is based on genome sequence information and LC-MS/MS analysis and aims to facilitate identification of molecules from complex microbial and plant extracts. Medicine A Training Program Using an Agility Ladder for Community-Dwelling Older Adults Vivian Castillo de Lima*1, Luz Albany Arcila Castaño*1, Vanessa Vilas Boas*1,2, Marco Carlos Uchida*1 1Applied Kinesiology Laboratory, School of Physical Education, University of Campinas, UNICAMP, 2Centro Universitário das Faculdades Associadas de Ensino - FAE The aim of this study is to present an agility training program for older people. The feasibility of this program is described, and the training protocol demonstrated by video imaging. Chemistry Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes Gabriela F. P. de Souza1, Airton G. Salles1 1Department of Organic Chemistry, Institute of Chemistry, University of Campinas A persulfate-promoted metal-free benzannulation of α,β-unsaturated compounds and alkynes in water toward the synthesis of unprecedented polyfunctionalized benzenes is reported. Environment A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions Carolina C. Monte-Bello*1,2, Elias F. Araujo*1,3, Marina C.M. Martins1, Valeria Mafra4, Viviane C.H. da Silva1,2, Viviane Celente1, Camila Caldana1,5 1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE, CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.
