2 articles published in JoVE
3D Modeling of Dendritic Spines with Synaptic Plasticity Gabriela Antunes*1, Fabio M. Simoes de Souza*1 1Center for Mathematics, Computation and Cognition, Federal University of ABC The protocol develops a three-dimensional (3D) model of a dendritic segment with dendritic spines for modeling synaptic plasticity. The constructed mesh can be used for computational modeling of AMPA receptor trafficking in the long-term synaptic plasticity using the software program Blender with CellBlender and MCell.
Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products Renata Sigrist1, Bruno S. Paulo1, Célio F. F. Angolini2, Luciana G. De Oliveira1 1Department of Organic Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), 2Center for Natural and Human Sciences, Federal University of ABC (UFABC) A mass spectrometry-guided genome mining protocol is established and described here. It is based on genome sequence information and LC-MS/MS analysis and aims to facilitate identification of molecules from complex microbial and plant extracts.