Universidade de Sao Paulo
3 articles published in JoVE
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells Guilherme Henrique Gatti da Silva1, Patricia P. Coltri1 1Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo We developed an intronic microRNA biogenesis reporter assay to be used in cells in vitro with four plasmids: one with intronic miRNA, one with the target, one to overexpress a regulatory protein, and one for Renilla luciferase. The miRNA was processed and could control luciferase expression by binding to the target sequence.
Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae Fernando Gomes1, Helena Turano1, Angélica Ramos1, Luis E. S. Netto1 1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.
Positron Emission Tomography Imaging for In Vivo Measuring of Myelin Content in the Lysolecithin Rat Model of Multiple Sclerosis Daniele de Paula Faria1, Caroline Cristiano Real1, Larissa Estessi de Souza1, Alexandre Teles Garcez2, Fabio Luis Navarro Marques1, Carlos Alberto Buchpiguel1 1Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina, Universidade de Sao Paulo, 2Nuclear Medicine Division, Hospital Sirio-Libanes This protocol has the aim of monitoring in vivo myelin changes (demyelination and remyelination) by positron emission tomography (PET) imaging in an animal model of multiple sclerosis.