Universidade de Sao Paulo

3 articles published in JoVE

• Cancer Research

  
  A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells Guilherme Henrique Gatti da Silva¹, Patricia P. Coltri^{1 1}Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo We developed an intronic microRNA biogenesis reporter assay to be used in cells in vitro with four plasmids: one with intronic miRNA, one with the target, one to overexpress a regulatory protein, and one for Renilla luciferase. The miRNA was processed and could control luciferase expression by binding to the target sequence.

• Biochemistry

  
  Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae Fernando Gomes¹, Helena Turano¹, Angélica Ramos¹, Luis E. S. Netto^{1 1}Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.

• Neuroscience

  
  Positron Emission Tomography Imaging for In Vivo Measuring of Myelin Content in the Lysolecithin Rat Model of Multiple Sclerosis Daniele de Paula Faria¹, Caroline Cristiano Real¹, Larissa Estessi de Souza¹, Alexandre Teles Garcez², Fabio Luis Navarro Marques¹, Carlos Alberto Buchpiguel^{1 1}Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina, Universidade de Sao Paulo, ²Nuclear Medicine Division, Hospital Sirio-Libanes This protocol has the aim of monitoring in vivo myelin changes (demyelination and remyelination) by positron emission tomography (PET) imaging in an animal model of multiple sclerosis.