Universidad Catolica del Norte 1 article published in JoVE Neuroscience Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor Jeanette Wihan1, Isabell Karnatz1, Isabelle Sébastien1, Ralf Kettenhofen1, Benjamin Schmid2, Christian Clausen2, Benjamin Fischer1, Rachel Steeg3, Heiko Zimmermann1,4,5,6, Julia C. Neubauer1,4 1Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology, Saarland University, 6Facultad de Ciencias del Mar, Universidad Católica del Norte This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.
