Sichuan University View Institution's Website 17 articles published in JoVE Medicine Gasless Endoscopic Thyroidectomy via the Trans-Axillary Approach Xun Zheng1, Xiaofei Wang1, Tao Wei1 1Division of Thyroid Surgery, Department of General Surgery, West China Hospital, Sichuan University This protocol presents the procedure for conducting thyroidectomy using the gasless endoscopic thyroidectomy trans-axillary approach (GETTA). Medicine Establishment of an Oronasal Fistula Mice Model Jiali Chen1, Jiayi Yin1, Shiming Zhang1, Shiyong Zhuang2, Renjie Yang3, Yichen Xu4, Qian Zheng1, Bing Shi1, Hanyao Huang1 1State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Department of Oral Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, 2State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, 3State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Eastern Clinic, West China Hospital of Stomatology, Sichuan University, 4State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Department of Oral Prosthodontics, West China Hospital of Stomatology, Sichuan University This article outlines a step-by-step procedure for establishing a mice model with an oronasal fistula. The oronasal fistula was created by employing heated ophthalmologic cautery to damage the midline portion of the hard palate, resulting in the formation of an opening between the oral and nasal cavities. Medicine Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy Yiming Song*1, Peiran Li*2, Ping Lyu1, Yanshen Yu1, Xinyu Chen1, Chen Cui3, Ruiye Bi2, Yi Fan1 1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, 2State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthognathic and TMJ Surgery, West China Hospital of Stomatology, 3Guangdong Province Key Laboratory of Stomatology, Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University An animal model of acquired hypoparathyroidism (HypoPT) is crucial to understanding how HypoPT affects mineral ion homeostasis and to verify the effectiveness of novel treatments. Here, a technique is presented to generate an acquired hypoparathyroidism (AHypoPT) rat model by parathyroidectomy (PTX) using carbon nanoparticles. Cancer Research Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma Maodi Wu1,3, Yang Liu2, Yi Zhao3, Yuyao Zhang4, Liansi Huang3, Qijun Du3, Ting Zhang1,3, Zhihui Zhong1,3, Han Luo2, Kai Xiao1,3 1Precision Medicine Research Center, Sichuan Provincial Key Laboratory of Precision Medicine, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, 2Department of Thyroid Surgery, West China Hospital, Sichuan University, 3Sichuan Kangcheng Biotech Co., 4Sichuan Cancer Hospital The present protocol establishes and characterizes a patient-derived xenograft (PDX) model of anaplastic thyroid carcinoma (ATC) and head and neck squamous cell carcinoma (HNSCC), as PDX models are rapidly becoming the standard in the field of translational oncology. Medicine Transoral Endoscopic Thyroidectomy Vestibular Approach for Thyroid Lobectomy Zhujuan Wu*1, Ye Tian*2, Ziwei Chen1, Anping Su2 1West China School of Medicine, Sichuan University, 2Sichuan University West China Hospital Here, we present a protocol to describe the methodology for the transoral endoscopic thyroidectomy vestibular approach in detail. Medicine A Personalized 3D-Printed Model for Preoperative Evaluation in Thyroid Surgery Pengyu Li*1, YIFei Chen*2, Wanjun Zhao1, Zhimian Huang3, Jingqiang Zhu1 1Department of Thyroid Surgery, West China Hospital, Sichuan University, 2West China School/Hospital of Stomatology, Sichuan University, 3Encomomic School of New York University Shanghai Here, a new method of establishing a personalized 3D-printed model for preoperative evaluation of thyroid surgery is proposed. It is conducive to preoperative discussion, reducing the difficulty of thyroid surgery. Cancer Research Spontaneous Murine Model of Anaplastic Thyroid Cancer Huayun Yan1, Yingfang Ma1, Xinyue Zhou2, Yushuang He3, Yang Liu2, Carlos Caulin4, Leiming Wang5, Heng Xu1,6, Han Luo2,6 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, 2Division of Thyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-Related Molecular Network, Department of General Surgery, West China Hospital, Sichuan University, 3Department of Ultrasound, West China Hospital of Sichuan University, Sichuan University, 4Department of Otolaryngology-Head & Neck Surgery and University of Arizona Cancer Center, University of Arizona, 5Center for Translational Medicine, Shenzhen Bay Laboratory, 6Division of Laboratory Medicine/Research Centre of Clinical Laboratory Medicine, West China Hospital, Sichuan University Here, we present a standard pipeline to obtain murine ATC tumors by spontaneous genetically engineered mouse models. Further, we present tumor dynamics and pathological information about the primary and metastasized lesions. This model will help researchers to understand tumorigenesis and facilitate drug discoveries. Biology The Establishment of a Murine Mandibular Molar Extraction Socket Healing Model Changhao Yu1,2, Fanyuan Yu1,2, Feifei Li1,2, Ling Ye1,2 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, 2Department of Endodontics, West China Stomatology Hospital, Sichuan University This protocol demonstrates step-by-step details of how to extract the mandibular first molar in the mouse. It provides an alternative method for researchers focusing on jawbone healing and regeneration. Cancer Research High-Resolution Ultrasonography for the Analysis of Orthotopic ATC Tumors in a Genetically Engineered Mouse Model Yushuang He1, Yan Luo1, Zhihui Li2, Han Luo2, Huayun Yan3, Yingfang Ma3, Xinyue Zhou2 1Department of Ultrasound, West China Hospital, Sichuan University, 2Department of Thyroid and Parathyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, 3State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center The present protocol describes high-frequency ultrasonography for visualizing the entire mouse thyroid gland and monitoring the growth of anaplastic thyroid carcinoma. Biochemistry A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors Lin Cheng*1, Lantian Su*1, Xiaowen Tian*1, Fan Xia2, Chang Zhao1, Wei Yan1, Zhenhua Shao1 1Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, 2Department of Neurosurgery, West China Hospital, Sichuan University S1P exerts its diverse physiological effects through the S1P receptors (S1PRs) subfamily. Here, a pipeline is described to expound on the structures and function of S1PRs. Neuroscience A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid Ling-Yi Liao1,2,3, Ying-Qian Zhang4, Benson Wui-Man Lau5, Qiong Wu6, Zi-Yan Fan1,3, Qiang Gao1,3, Zhi-Hui Zhong6,7 1Department of Rehabilitation Medicine, West China Hospital, Sichuan University, 2Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, 3Key Laboratory of Rehabilitation Medicine in Sichuan Province, West China Hospital, Sichuan University, 4Department of physiology, Southwest Medical University, 5Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, 6Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 7Sichuan Kangcheng Biotech Co., Inc. Here, we describe the procedure for a pilot study to explore the effect of repetitive transcranial magnetic stimulation with different frequencies (1 Hz/20 Hz/40 Hz) on Aβ and tau metabolism in rhesus monkey cerebrospinal fluid. Cancer Research Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane Moe Ishihara*1, Junhui Hu*1, Xiaoyu Zhang2, YongHyeon Choi3, Anthony Wong4, Celine Cano-Ruiz5, Rongwei Zhao6, Ping Tan7, Jonathan L. Tso1, Lily Wu1,8 1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Bioengineering, Hanyang University, 4Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 6School of Life Sciences, Beijing Normal University, 7Department of Urology, West China Hospital, Sichuan University, 8Department of Urology, David Geffen School of Medicine, University of California, Los Angeles Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. This protocol illustrates two novel animal models for the disease: the orthotopically implanted mouse model and the chicken chorioallantoic membrane model, both of which demonstrate lung metastasis resembling clinical cases. Immunology and Infection Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression Zhihan Wang*1,2, Qinqin Pu*1,2, Ping Lin*2,3, Changlong Li1, Jianxin Jiang3, Min Wu2 1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis. Engineering An Available Technique for Preparation of New Cast MnCuNiFeZnAl Alloy with Superior Damping Capacity and High Service Temperature Dong Li1,3, Wenbo Liu1,2, Ning Li1, Zhenyu Zhong1, Jiazhen Yan1, Sanqiang Shi2 1School of Manufacturing Science and Engineering, Sichuan University, 2Department of Mechanical Engineering, Hong Kong Polytechnic University, 3Department of Mechanical and Electrical Engineering, Chengdu Aeronautic Polytechnic Here we present a protocol to obtain a novel Mn-Cu-based alloy with excellent comprehensive performances by a high-quality smelting technology and reasonable heat treatment methods. Neuroscience A Rat Model of Central Fatigue Using a Modified Multiple Platform Method Weiyue Zhang*1, Wei Zhang*1, Ning Dai*1, Chenxia Han2, Fengzhi Wu1, Xu Wang1, Libo Tan1, Jie Li1, Feng Li1, Qingjia Ren3 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, 3Institute of Tibetan Medicine, Tibetan Traditional Medical College Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM). Medicine Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation Ruiye Bi1,2, Yi Fan2,3, En Luo2,4, Quan Yuan2,4, Michael Mannstadt1 1Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, 2West China School of Stomatology, Sichuan University, 3Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, 4State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor. Immunology and Infection An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci Marlise I. Klein1, Jin Xiao1,2, Arne Heydorn3, Hyun Koo1,4 1Center for Oral Biology, University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases, Sichuan University, 3Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology, University of Rochester Medical Center Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.