The Third Affiliated Hospital of Sun Yat-sen University 4 articles published in JoVE Medicine The Examination of Peroxidase-Positive Leukocytes in Semen Xiaoming Li*1, Xiaohua Cai*1, Liuhong Cai1, Jianping Ou1 1Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University This paper presents an economical and efficient protocol for examining peroxidase-positive leukocytes in semen. With the assistance of a computer-assisted semen analysis (CASA) system, the concentration of peroxidase-positive leukocytes in semen can be obtained within a total of 60 min, effectively improving the efficiency of andrology laboratory and andrologists. Medicine Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles Yuan Sang*1, Junbin Zhang*1, Chang Liu1, Kaihua Liu1, Hui Yao1, Huiqing Zhao1, Wenbin Xu1, Yichun Xu1, Gang Hou1 1Department of Orthopedics, The Third Affiliated Hospital of Sun Yat-sen University Presented here is a protocol for using silver nanoparticles to effectively ameliorate the acute symptoms of type II collagenase-induced osteoarthritis mice, including synovial inflammation, synovial hyperplasia, vascular hyperplasia, etc. Neuroscience Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions Benedetta Assetta*1, Changyong Tang*1,2, Jing Bian*3, Ryan O'Rourke1, Kevin Connolly1, Thomas Brickler3, Sundari Chetty3,4, Yu-Wen Alvin Huang1,5,6 1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University The neuron-glial interactions in neurodegeneration are not well understood due to inadequate tools and methods. Here, we describe optimized protocols to obtain induced neurons, oligodendrocyte precursor cells, and oligodendrocytes from human pluripotent stem cells and provide examples of the values of these methods in understanding cell-type-specific contributions in Alzheimer’s disease. Developmental Biology A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats Shihai Jiang*1, Xiaoyou Yi*3, Yuansen Luo2, Dongjie Yu1, Yuangao Liu1, Fei Zhang1,4, Lei Zhu2, Kun Wang1 1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.
