The Second Affiliated Hospital of Guangzhou University of Chinese Medicine 6 articles published in JoVE Medicine Management of the Uncinate Process in No-Touch Laparoscopic Pancreaticoduodenectomy Zhantao Shen1,2, Xiang Wu1,2, Fanxing Huang2, Guihao Chen1,2, Yifeng Liu1,2, Zhimin Yu1,2, Chunbao Zhu1,2, Zhijian Tan1,2, Xiaosheng Zhong1,2 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine Complete resection of the uncinate process and mesopancreas is one of the most important and difficult processes in laparoscopic pancreatoduodenectomy (LPD). This article presents a method for managing the uncinate process in no-touch LPD using the median-anterior and left-posterior approaches to the superior mesenteric artery (SMA). Medicine Fu's Subcutaneous Needling for Knee Osteoarthritis Pain Po-En Chiu*1,2, Zhonghua Fu*3,4, Jian Sun4,5,6, Guan-Wei Jian7,8, Te-Mao Li9, Li-Wei Chou10,11,12 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills. Neuroscience Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients Songjun Lin1,2, Qiang Lin3,4,5, Biyi Zhao3,4,6, Yongchun Jiang3,4, Wanqi Zhuang3,4, Delong Chen3,4, Yajie Zhang3,4, Aijia Chen3,4, Qianrong Zhang7, Yuxin Zheng3,4, Jianjun Wang1, Fangqiu Xu8, Xi Qin3,4,9, Yefeng Cai10 1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients. Medicine Laparoscopic Radical Antegrade Modular Pancreatosplenectomy via Dorsal-Caudal Artery Approach for Pancreatic Neck-Body Cancer Zhantao Shen1,2, Guihao Chen1,2, Chunbao Zhu2, Xiang Wu1,2, Yifeng Liu1,2, Zhangyuanzhu Liu1,2, Zhijian Tan1,2, Xiaosheng Zhong1,2 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine With advancements in laparoscopic techniques, laparoscopic radical antegrade modular pancreatosplenectomy (L-RAMPS) has been widely recognized. However, owing to several technical difficulties in this procedure, the artery-first approach in L-RAMPS still remains uncommon. Here, we developed the dorsal-caudal artery approach for L-RAMPS, which might be safe and beneficial for pancreatic neck tumors. Genetics Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model Yu Xia*1,2, Jinlin Hu*3, Xiang Li4, Shuang Zheng4, Ge Wang1,2, Songtao Tan1,2, Zengxiao Zou1,2, Qiong Ling2,5, Fenghua Yang4, Xiaoping Fan1,2 1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation. Medicine Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique Zhantao Shen1,2, Xiang Wu1,2, Guihao Chen1,2, Yifeng Liu1,2, Chunbao Zhu2, Fanxing Huang2, Zhijian Tan1,2, Xiaosheng Zhong1,2 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine No-Touch isolation procedures might prevent the dissemination of cancer cells from the primary tumor. However, these techniques are not widely accepted in laparoscopic pancreatoduodenectomy (LPD) by now. We herein present in-situ No-Touch isolation LPD with partial resection and reconstruction of the superior mesenteric vein (SMV) for pancreatic cancer after neoadjuvant therapy.