Capital Medical University, National Center for Children's Health

3 articles published in JoVE

• Immunology and Infection

  
  Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children Huizheng Ge*¹, Linlin Zhang*^2,3, Mengjia Liu*^2,3, Xi Yang¹, Xu Wu¹, Ran Wang^2,3, Zhengde Xie^{2,3 1}Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, ² In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.

• Immunology and Infection

  
  Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique Linlin Zhang*^1,2, Meng Zhang*^1,2,3, Mengjia Liu^1,2, Junhong Ai^1,2, Jiao Tian^1,2, Huizheng Ge⁴, Ran Wang^1,2, Zhengde Xie^{1,2 1} The present protocol combines ex vivo stimulation and flow cytometry to analyze polyfunctional T cell (TPF) profiles in peripheral blood mononuclear cells (PBMCs) within Japanese encephalitis virus (JEV)-vaccinated children. The detection method and flow cytometry color scheme of JEV-specific TPFs were tested to provide a reference for similar studies.

• Immunology and Infection

  
  Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis Linlin Zhang^1,2, Mengjia Liu^1,2, Meng Zhang^1,2, Junhong Ai^1,2, Jiao Tian^1,2, Ran Wang^1,2, Zhengde Xie^{1,2 1} We describe a method combining immunomagnetic beads and fluorescence-activated cell sorting to isolate and analyze defined immune cell subpopulations of peripheral blood mononuclear cells (monocytes, CD4⁺ T cells, CD8⁺ T cells, B cells, and natural killer cells). Using this method, magnetic and fluorescently labeled cells can be purified and analyzed.