Yamaguchi University 2 articles published in JoVE Bioengineering Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype Kinya Matsuo1, Britta Engelhardt2, Hideaki Nishihara3 1Graduate School of Medicine, Yamaguchi University, 2Theodor Kocher Institute, University of Bern, 3Department of Neurotherapeutics, Yamaguchi University of Medicine Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro. Behavior Four Temporary Waterslide Designs Adapted to Different Slope Conditions to Encourage Child Socialization in Playgrounds Zhudi Hua1, Ting Tao1, Risa Akita1, Tomofusa Akita1, Yoshiaki Hayakawa1, Masanori Hariyama2, Hayato Sakurai3, Ricki Colman4, Mamiko Koshiba1,2,3 1Graduate School of Sciences and Technology for Innovation, Yamaguchi University, 2Graduate School of Information Sciences, Tohoku University, 3Pediatrics, Saitama Medical University Hospital, 4Department of Cell and Regenerative Biology, University of Wisconsin-Madison Early life social learning is enhanced by interactions with effectively designed environments. Four events were held at different city parks using inexpensive, temporary waterslides to stimulate social learning. This study describes the prototypes used and the evaluation of the children's interactions.