Chungnam National University 7 articles published in JoVE Biochemistry Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue Sanghun Lee*1, Gyumin Lim*2,3, Soyeon Kim*1, Hyoju Kim1, Yeon Jin Roh3, Wantae Kim2, Dong Wook Choi3, Su Myung Jung1 1Department of Biological Sciences, Sungkyunkwan University (SKKU), 2Department of Biochemistry, College of Natural Sciences, Chungnam National University, 3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University In this protocol, methods relevant for BAT-optimized arteriovenous metabolomics using GC-MS in a mouse model are outlined. These methods allow for the acquisition of valuable insights into BAT-mediated metabolite exchange at the organismal level. Neuroscience In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy Eun-Seo Cho1, Seungjae Han1, Gyuri Kim1, Minho Eom1, Kang-Han Lee2, Cheol-Hee Kim2, Young-Gyu Yoon1,3 1School of Electrical Engineering, KAIST, 2Department of Biology, Chungnam National University, 3KAIST Institute for Health Science and Technology Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization. Neuroscience Burn Injury-Induced Pain and Depression-Like Behavior in Mice Jae-Gyun Choi*1, Dong-Wook Kang*1, Jaehyuk Kim1, Miae Lee1, Sheu-Ran Choi2, Jin Bong Park1, Hyun-Woo Kim1 1Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, 2Department of Pharmacology, Catholic Kwandong University College of Medicine A transient scald injury (65 °C ± 0.5 °C, 3 s) of one hind paw decreases the threshold (g) to von Frey filament stimulation of the ipsilateral side and alters gait pattern. Besides, burn injury induces depression-like behavior in the forced swimming test. Cancer Research Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells Jina Lee*1,2, Joo-Eun Lee*3, Seil Kim1,4,5, Dukjin Kang1, Hee Min Yoo1 1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST) Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways. Genetics An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness Seoghyun Lee*1, Minho Won*2, Ran Hee Hwang3, Gang Min Hur2, Hyunju Ro1 1Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, 2Department of Pharmacology, College of Medicine, Chungnam National University, 3 Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment. Neuroscience Automated Gait Analysis in Mice with Chronic Constriction Injury Dong-Wook Kang1, Jae-Gyun Choi1, Ji-Young Moon2, Suk-Yun Kang2, Yeonhee Ryu2, Jin Bong Park1, Hyun-Woo Kim1,3 1Department of Physiology and Medical Science, College of Medicine and Brain Research Institute, Chungnam National University, 2KM Fundamental Research Division, Korea Institute of Oriental Medicine (KIOM), 3Department of Neuroscience and Cell Biology, University of Texas Medical Branch at Galveston The precise assessment of pain response in a neuropathic animal model is critical to investigate the pathophysiology of pain diseases and develop new analgesics. We present a sensitive and objective method to determine the sensory function of the rodent hind paw by an automated gait analysis system. Cancer Research Ultrasonographic Evaluation of Breast Cancer-related Lymphedema Yumi Jeon1, Jaewon Beom1, Soyoung Ahn1, Soo-Kyung Bok1 1Department of Rehabilitation Medicine, Chungnam National University Hospital (CNUH) This study utilized ultrasonography for diagnosis and follow-up tests to confirm treatment efficacy of Progressive Resistance Exercise (PRE) in breast cancer-related lymphedema (BCRL). Ultrasonography can be applied effectively to ensure diagnosis and treatment of lymphedema.
