Ulsan National Institute of Science and Technology (UNIST) 6 articles published in JoVE Chemistry Synthesis of Single-Crystalline Core-Shell Metal-Organic Frameworks Jihyun Park1, Junsu Ha1, Hoi Ri Moon1 1Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST) Here, we demonstrate a protocol for the two-step synthesis of single-crystalline core-shells using a non-isostructural metal-organic framework (MOF) pair, HKUST-1 and MOF-5, which have well-matched crystal lattices. Biochemistry Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique Yujin Kang1, Subin Bae1, Soyeong An1, Ja Yil Lee1,2 1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS) DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase. Biology Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis Chi-Ju Kim*1,2, Morgan D. Kuczler*1, Liang Dong1,3, Junyoung Kim2,4, Sarah R. Amend1, Yoon-Kyoung Cho2,4, Kenneth J. Pienta1 1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS) Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device. Chemistry Key Factors Affecting the Performance of Sb2S3-sensitized Solar Cells During an Sb2S3 Deposition via SbCl3-thiourea Complex Solution-processing Yong Chan Choi1, Sangl Il Seok2, Eunjeong Hwang1, Dae-Hwan Kim1 1Convergence Research Center for Solar Energy, Daegu Gyeongbuk Institute of Science and Technology (DGIST), 2Perovtronics Research Center, School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST) This work provides a detailed experimental procedure for the deposition of Sb2S3 on a mesoporous TiO2 layer using a SbCl3-thiourea complex solution for applications in Sb2S3-sensitized solar cells. This article also determines key factors governing the deposition process. Environment Biofilm Removal Using Carbon Dioxide Aerosols without Nitrogen Purge Seongkyeol Hong1, Jaesung Jang1 1School of Mechanical and Nuclear Engineering, Ulsan National Institute of Science and Technology (UNIST) Biofilms on surfaces can be effectively and rapidly removed by using a periodic jet of carbon dioxide aerosols without a nitrogen purge. Bioengineering Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood Yang-Seok Park1, Vijaya Sunkara1, Yubin Kim1, Won Seok Lee1,2, Ja-Ryoung Han1,3, Yoon-Kyoung Cho1,4 1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3KOGAS (Korea Gas Corporation) Research Institute, 4Center for Soft and Living Matter, Institute for Basic Science (IBS) This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.
