Hubrecht Institute 1 article published in JoVE Biology Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids Marie Bannier-Hélaouët1,2, Maarten H. Geurts1,2, Jeroen Korving1, Harry Begthel1, Hans Clevers1,2 1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), University Medical Center Utrecht, 2Oncode Institute, Hubrecht Institute This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.