Jagiellonian University Medical College 3 articles published in JoVE Immunology and Infection Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines Aneta Babula*1,2, Izabela Siemińska*1,3, Monika Baj-Krzyworzeka1 1Department of Clinical Immunology, Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences, Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU, University of Agriculture in Kraków The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them. Immunology and Infection Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis Magdalena Zemelka-Wiacek1, Monika Majewska-Szczepanik2, Pawel Gajdanowicz1, Marian Szczepanik3 1Department of Clinical Immunology, Wroclaw Medical University, 2Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, 3Chair of Biomedical Sciences, Faculty of Health Sciences, Jagiellonian University Medical College Contact hypersensitivity (CHS) is a murine experimental model of allergic contact dermatitis (ACD). CHS is based on sensitization with reactive hapten by painting the shaved skin of the chest and abdomen, with a subsequent ear skin challenge with a diluted hapten, causing a swelling reaction that is assessed in various ways. Biology Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation Gabriela Gorczyca1, Kamil Wartalski2, Zbigniew Tabarowski3, Malgorzata Duda1 1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow, 2Department of Histology, Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow Presented here are two protocols for the encapsulation of porcine oocytes in 3D culture conditions. In the first, cumulus-oocyte complexes (COCs) are encapsulated in fibrin-alginate beads. In the second, they are enclosed with fluorinated ethylene propylene powder particles (microbioreactors). Both systems ensure optimal conditions to maintain their 3D organization.
