Barrow Neurological Institute View Institution's Website 6 articles published in JoVE Neuroscience In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice Rocio Leal-Campanario1,2, Susana Martinez-Conde2,3, Stephen L. Macknik2,3 1División de Neurociencias, Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice. Neuroscience Simultaneous Cryosectioning of Multiple Rodent Brains Tabitha R.F. Green1,2,3, J. Bryce Ortiz1, Jordan L. Harrison5, Jonathan Lifshitz1,3,4, Rachel K. Rowe1,3,4 1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging. Neuroscience Fiber Connections of the Supplementary Motor Area Revisited: Methodology of Fiber Dissection, DTI, and Three Dimensional Documentation Baran Bozkurt1, Kaan Yagmurlu2, Erik H. Middlebrooks3, Zuzan Cayci4, Orhun M. Cevik1, Ali Karadag5, Sean Moen1, Necmettin Tanriover6, Andrew W. Grande1 1Department of Neurosurgery, University of Minnesota, 2Department of Neurosurgery, Barrow Neurological Institute, St. Josephs Hospital and Medical Center, 3Department of Radiology, University of Alabama at Birmingham, 4Department of Radiology, University of Minnesota, 5Department of Neurosurgery, Tepecik Training and Research Hospital, 6Department of Neurosurgery, Cerrahpasa Medical School, University of Istanbul The purpose of this study is to show each step of the fiber dissection technique on human cadaveric brains, the 3D documentation of these dissections, and the diffusion tensor imaging of the anatomically dissected fiber pathways. Bioengineering PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS Rebecca L. McCall1, Rachael W. Sirianni1 1Barrow Brain Tumor Research Center, Barrow Neurological Institute We describe the production and characterization of nanoparticles and microparticles composed of poly(lactic-co-glycolic acid) using vitamin E-TPGS as an emulsifier. By varying formulation parameters such as the concentration of emulsifier, it is possible to produce nanoparticles with mean diameters ranging from 220 nm to 1.98 µm. Application Notes 鼠脑胶质瘤的颅内植入和3维体内生物发光成像 - ADVERTISEMENT Mohammed G. Abdelwahab1, Tejas Sankar2, Mark C. Preul2, Adrienne C. Scheck1,2 1Neuro-Oncology Research, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center Medicine Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas Mohammed G. Abdelwahab1, Tejas Sankar2, Mark C. Preul2, Adrienne C. Scheck1,2 1Neuro-Oncology Research, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center, 2Neurosurgery Research Laboratory, Barrow Neurological Institute of St. Joseph’s Hospital and Medical Center Intracranial implantation of GL261 cells into C57BL/6 mice produces malignant gliomas that recapitulate many of the hallmarks of human glioblastoma multiforme. We used GL261 cells stably expressing luciferase to allow us to use in vivo imaging to follow tumor progression. The surgery and 3D in vivo imaging are demonstrated.
