Mayo Clinic Florida 4 articles published in JoVE Medicine Point-Of-Care Ultrasound Screening for Proximal Lower Extremity Deep Venous Thrombosis Rebecca G. Theophanous1, Vinca W. Chow2, David L. Convissar3, Stephen C. Haskins4,5, Robert A. Jones6, Hari K. P. Kalagara7, Yuriy S. Bronshteyn8 1Department of Emergency Medicine, Duke University School of Medicine, 2Department of Anesthesiology, Dartmouth-Hitchcock Medical Center, 3Department of Anesthesiology, Massachusetts General Hospital, 4Department of Anesthesiology, Critical Care and Pain Management, Hospital for Special Surgery, 5Department of Anesthesiology, Weill-Cornell Medical College, 6Department of Emergency Medicine, MetroHealth Medical Center/Case Western Reserve University, 7Department of Anesthesiology, Mayo Clinic, 8Department of Anesthesiology, Duke University School of Medicine Traditionally, lower extremity deep venous thrombosis (DVT) is diagnosed by radiology-performed venous duplex ultrasound. Providers appropriately trained in focused point-of-care ultrasound (POCUS) can perform a rapid bedside examination with high sensitivity and specificity in critically ill patients. We describe the scanning technique for focused POCUS DVT lower extremity examination. Behavior Assessing Dominant-Submissive Behavior in Adult Rats Following Traumatic Brain Injury Dmitry Frank*1, Benjamin F. Gruenbaum*2, Michael Semyonov1, Yair Binyamin1, Olena Severynovska3, Ron Gal1, Amit Frenkel1, Boris Knazer4, Matthew Boyko1, Alexander Zlotnik1 1Division of Anesthesiology and Critical Care, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, 3Department of Biochemistry and Physiology of the Faculty of Biology and Ecology, Oles Gonchar of the Dnipro National University, 4Department of Ophthalmology, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev The present protocol describes a rat model of fluid percussion-induced traumatic brain injury followed by a series of behavioral tests to understand the development of dominant and submissive behavior. Using this model of traumatic brain injury in conjunction with specific behavioral tests enables the study of social impairments following brain injury. Neuroscience A Metric Test for Assessing Spatial Working Memory in Adult Rats Following Traumatic Brain Injury Dmitry Frank*1, Benjamin F. Gruenbaum*2, Israel Melamed3, Julia Grinshpun1, Yair Benjamin1, Ievgeni Vzhetson1, Nadia Kravchenko4, Michael Dubilet1, Matthew Boyko*1, Alexander Zlotnik*1 1Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 2Department of Anesthesiology and Perioperative Medicine, Mayo Clinic, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 4Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University Traumatic brain injury (TBI) is commonly associated with memory impairment. Here, we present a protocol to assess spatial working memory after TBI via a metric task. A metric test is a useful tool to study spatial working memory impairment after TBI. Cancer Research Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture Alicia K. Fleming Martinez1, Peter Storz1 1Department of Cancer Biology, Mayo Clinic Florida Primary acinar cell isolation, protein expression or activity modulation, culture, and down-stream applications are abundantly useful in the ex vivo study of acinar-to-ductal metaplasia (ADM), an early event in the development of pancreatic cancer.