Human Genetics Institute of New Jersey 1 article published in JoVE Developmental Biology Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes Mansour Aboelenain1,2,3, Karen Schindler1,2, Cecilia S. Blengini1,2 1Department of Genetics, Rutgers, The State University of New Jersey, 2Human Genetics Institute of New Jersey, 3Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University Error in chromosome segregation is a common feature in oocytes. Therefore, studying the spindle assembly checkpoint gives important clues about the mechanisms needed to produce healthy eggs. The present protocol describes three complementary assays to evaluate spindle assembly checkpoint integrity in mouse oocytes.
