Hauptman-Woodward Medical Research Institute 1 article published in JoVE Biochemistry High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography Gabrielle R. Budziszewski1, M. Elizabeth Snell1, Tiffany R. Wright1, Miranda L. Lynch1, Sarah E. J. Bowman1,2 1National High-Throughput Crystallization Center, Hauptman-Woodward Medical Research Institute, 2Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, The State University of New York This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.
