Universidad Nacional de Colombia 2 articles published in JoVE Developmental Biology Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes Daniela Londoño-Vásquez1,2, Alexander Jurkevich3, Ahmed Z. Balboula1 1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser. Bioengineering Electric and Magnetic Field Devices for Stimulation of Biological Tissues Juan José Saiz Culma1,2, Juan Felipe Escobar Huertas2,3, Diego Alexander Garzón-Alvarado1,2, Juan Jairo Vaca-Gonzalez1,4 1Biomimetics Laboratory, Instituto de Biotecnología, Universidad Nacional de Colombia, 2Numerical Methods and Modeling Research Group (GNUM), Universidad Nacional de Colombia, 3Design, Analysis and Development of Engineering Systems Research group (GIDAD), Fundación Universitaria Los Libertadores, 4School of Health and Sports Sciences, Master Program in Epidemiology, Fundación Universitaria del Área Andina This protocol describes the step-by-step process to build both electrical and magnetic stimulators used to stimulate biological tissues. The protocol includes a guideline to simulate computationally electric and magnetic fields and manufacture of stimulator devices.
