Jove Lab Bio

Lab 12: DNA Isolation and Restriction Enzyme Analysis — Materials List

Preparation for 10 Students

• Popsicle sticks (1/group)
  5
• Glass funnels (1/group)
  5
• 15 mL conical tubes (2/group)
  10
• Drinking cups (1/group)
  5
• 1 % NaCl solution (10 mL in a drinking cup/group)
  50
• Permanent marker (1/group)
  5
• Beaker for collecting waste (1/group)
  5
• Lysis buffer I: TE buffer with SDS (TES Buffer) (1 mL/group)
  5 mL
• Lysis buffer II: TE buffer without SDS (1 mL/group)
  5 mL
• Proteinase K (10 µL/ group)
  50 µL
• 2.5 M NaCl (100 µL/group)
  500 µL
• Ethanol 100 % (4°C) (1 mL/group)
  5 mL
• Glass rods (2/group)
  10
• Stopwatch (1/group)
  10
• Microfuge tubes (1.5 mL) (a beaker full/station)
  1 per station
• Floating foam microfuge racks (1/station)
  1 per station
• Cell phone with a camera
  1 per group
• Automated pippete sets (1 µL-1 mL)
  Dependent on the lab size
• Pipette tips (1 µL-1 mL) 1box each/group
  Dependent on the lab size
• Microfuge centrifuge
  Dependent on the lab size
• Water bath (set to 56°C)
  Dependent on the lab size
• Nuclease free water (125 µL aliquot/group)
  125 µL
• Lamda DNA (12 µL/group)
  12 µL
• NEB buffer 2.1 (15 µL/group)
  15 µL
• Restriction enzyme 1 (StuI) (1 µL/group)
  1 µL
• Restriction enzyme 2 (BsrGI) (1 µL/group)
  1 µL
• Stop watch (1/group)
  5
• Enzyme cooler
  Dependent on the lab size
• Water bath (set to 37°C)
  Dependent on the lab size
• Computer with internet connection
  Dependent on the lab size
• Agarose Powder
  Dependent on the lab size
• Tris-Acetate-EDTA buffer
  Dependent on the lab size
• Syber safe gel stain (10,000X) (10 µl/100mL gel)
  Dependent on the lab size
• 6X loading dye
  Dependent on the lab size
• 1 kb DNA Ladder
  Dependent on the lab size
• Weighing balance
  1 whole class
• Microwave
  1 whole class
• DNA gel apparatus
  1 whole class
• UV transilluminator
  1 whole class